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KMID : 0613820080180081049
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Journal of Life Science
2008 Volume.18 No. 8 p.1049 ~ p.1052
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Production of Cellulolytic Enzymes by Aspergillus niger on Solid and Submerged State Fermentation
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M. Subhosh Chandra
B. Rajasekhar Reddy
Choi Yong-Lark
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Abstract
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Microbial production of cellulolytic enzymes by Aspergillus niger in solid state fermentation (SSF) and submerged state fermentation (SF) in laboratory scale was compared. Czapek Dox liquid broth amended with cellulose (0.5%) was used for cultivation in SF, whereas rice bran was used as a solid support, moistened with cellulose, amended Czapek Dox broth for growth in SSF. The production of Carboxymethyl cellulase, Filter paperase and β-Glucosidase was monitored at regular intervals. The peak production of the enzymes occurred within 3 days of incubation in SSF as against ≥ 7 days in SF. SSF gave higher yields of enzymes in comparison to SF. Maximum titres of 0.40, 0.62 and 0.013 U/ml in respect of FPase, CMCase and β-glucosidase in SSF were recovered as against 0.13, 0.06 and 0.0013 U/ml in SF respectively, at their respective peak time intervals. Hence, SSF appeared to be a better choice for production of cellulolytic enzymes by Aspergillus niger.
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KEYWORD
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Aspergillus niger, submerged state fermentation, solid state fermentation, filter paperase, carboxymethyl cellulase, β-glucosidase
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